(a)
(b)
BASE_EXCISION_REPAIR
Enrichment plot: KEGG_BASE_EXCISION_REPAIR
CELL_CYCLE
MISMATCH_REPAIR
N
Enrichment score (ES)
0.0
NUCLEOTIDE_EXCISION_REPAIR
SPLICEOSOME
0.5
HOMOLOGOUS_RECOMBINATION
0,4
DNA_REPLICATION
0.3
RIBOSOME
PROTEASOME
02
OOCYTE_MEIOSIS
0.1
ONE_CARBON_POOL_BY_FOLATE
0.0
Terms
RNA_DEGRADATION
RNA_POLYMERASE
PROTEIN_EXPORT
STEROID_BIOSYNTHESIS
Ranked list metric (Signal2Noise)
TERPENOID_BACKBONE_BIOSYNTHESIS
PS3_SIGNALING_PATHWAY
1.00
T (postively canelated)
BASAL_TRANSCRIPTION_FACTORS
0.75
N_GLYCAN_BIOSYNTHESIS PYRIMIDINE_METABOLISM
0.50
0.25
Zere cross at 18320
AMINOACYL_TRNA_BIOSYNTHESIS
0.00
GLYOXYLATE_AND_DICARBOXYLATE_METABOLISM
0.35
BIOSYNTHESIS_OF_UNSATURATED_FATTY_ACIDS
0.50
“D’ (negatively correlated)
RIBOFLAVIN_METABOLISM
0
5,000
10,000
15,000
20,000
25,000
GLYCOSYLPHOSPHATIDYLINOSITOL_GPI ANCHOR_BIOSYNTHESIS
Rank in Ordered Dataset
0
5
10
15
20
Count
Enrichment profile -Hits
Ranking metric scores
(c)
(d)
(e)
Enrichment plot: KEGG_CELL_CYCLE
Enrichment plot: KEGG_DNA_REPLICATION
Enrichment plot: KEGG_HOMOLOGOUS_RECOMBINATION
0.7
0.9
Enrichment score (ES)
0.8
0.9
0.6
Enrichment score (ES)
0.7
0.5
0.0
Enrichment score (ES)
0.7
A
0.4
05
2
0.4
0.5
03
4
0.2
0.1
0.1
2
0.0
0.0
Ranked list metric (Signal2Noise)
Ranked list metric (Signal2Noise)
Ranked list metric (Signal2Noise)
1.00
T’ @positively conelated)
1.00
1’ (postively conelated)
0.75
0.75
1.0
(p mitively comel die d)
0.50
0.50
0.5
0.25
Zero cross at 16370
0.25
Zero cross at 16370
0.00
Zero cross af 16370
0.00
00
0.25
0.26
0.50
0’ (negatively comelated)
0.50
D’ (negatively comelated)
0.5
0’ (negatively correlate d)
@
5.000
10,000
15,000
20.000
25.000
0
5.000
10.000
15.000
20,000
25.000
Q
2.800
6.000
7,500
10.000 12.500 15.000 17.500 20,000 22.500 25,000
Rank in Ordered Dataset
Rank in Ordered Dataset
Rank in Ordered Dataset
Enrichment profile -Hits
Ranking metric scores
Enrichment profile -Hits
Ranking metric scores
Enrichment profile -Hits
Ranking metric scores
(f)
(g)
(h)
Enrichment plot: KEGG_MISMATCH_REPAIR
Enrichment plot: KEGG_NUCLEOTIDE_EXCISION_REPAIR
Enrichment plot: KEGG_SPLICEOSOME
0.9
0.8
Enrichment score (ES)
0.7
0.7
0.5
0.6
Enrichment score (ES)
Enrichment score (ES)
0.4
0.5
0.5
0.3
0.2
02
0.1
0.1
Q.0
.1
0.0
0.0
Ranked list metric (Signal2Noise)
Ranked list metric (Signal2Noise)
Ranked list metric (Signal2Noise)
1.00
Tipositively couelated)
1.0
(postively conelated)
1.00
F (postively canelated)
.75
0.50
0.25
0.5
.50
Zero cross at 16370
Zere cross at 16370
Zero cross at 18370
0.00
0.0
0.25
0.50
D’(negatively comelated)
0.5
D’ (negatively conelated)
0.50
” (negatively correlate &)
0
5.000
10,000
15.000
20.000
25.000
0
2.500
5,000
7.500
10.000 12.500 15,000 17.500 20.000 22.500 25.000
0
5.000
10,000
15,000
20.000
25,000
Rank in Ordered Dataset
Rank in Ordered Dataset
Rank in Ordered Dataset
Enrichment profile
- Hits
Ranking metric scores
Enrichment profile - Hits
Ranking metric scores
Enrichment profile -Hits
Ranking metric scores
Supplementary Figure S6. Gene set enrichment analysis (GSEA). (a-b) “base excision repair”, (c) “cell cycle”, (d) “DNA replication”, (e) “homologous recombination”, (f) “mismatch repair”, (g) “nucleotide excision repair” and (h) “spliceosome”. Only the listed 7 common functional gene sets enriched in ACC samples with ANLN highly expressed.