33952721

SUPPLEMENTARY FIGURES

Supplementary Figure 1. The violin plot of differential expression of m6A genes between localized and metastatic tumor.

100

p=0.002

80

p=0.116

Gene expression

60

40

p=0.581

p=0.224 p=0.602

p=0.499

20

p=0.012

p=0.14 p=0.447

p=0.102

p=0.339 P=0.784

☒

p=0.003

0

YTHDF2

YTHDC2

ALKBH5

METTL3

METTL14

WTAP

FTO

YTHDC1

ZC3H13

KIAA1429

HNRNPC

RBM15

YTHDF1

Supplementary Figure 2. Correlation analysis among m6A regulatory genes. The cross signal (X) indicates no statistical ☒ significance. Positive and negative correlations are shown in red and blue, respectively. A darker color of circle indicates a stronger correlation.

WTAP

RBM15

YTHDF2

HNRNPC

KIAA1429

ZC3H13

ALKBH5

YTHDF1

METTL14

FTO

METTL3

YTHDC2

YTHDC1

1

WTAP

1

☒

☒

0.43

☒

☒

☒

☒

☒

☒

☒

☒

☒

RBM15

1

0.53

0.6

☒

6 ☒

☒

☒

☒

☒

☒

☒

☒

0.8

YTHDF2

1

0.62

☒

0.38

0.42

☒

☒

☒

0.41

0.37

0.62

0.6

HNRNPC

1

☒

0.4

0.45

☒

☒

☒

☒

0.52 ☒

0.4

FTO

1

0.6

☒

0.38

☒

☒

☒

☒

0.41

KIAA1429

1

☒

☒

☒

☒

☒

☒

0.52

0.2

METTL3

1

0.4

☒

☒

☒

☒

0.46

0

ZC3H13

1

☒

☒

☒

☒

0.39

-0.2

YTHDC2

1

☒

☒

☒

☒

ALKBH5

1

☒

☒

☒

-0.4

YTHDF1

1

☒

0.47

-0.6

METTL14

1

0.67

-0.8

YTHDC1

1

-1

Supplementary Figure 3. The process of Lasso regression analysis.

A

3

3

2

2

B

55

50

00

5

w

OS

00

w

5

CAS

CAS

53

N

2

N

N

2

2

2

1

1

0.5

9.8

2

0.4

9.6

0.3

9.4

Coefficients

Partial Likelihood Deviance

0.2

9.2

0.1

9.0

3

0.0

8.8

8.6

1

-5

-4

-3

-2

-5

-4

-3

-2

Log Lambda

Log(2)

Supplementary Figure 4. The landscape of immune abundance of 22 leukocyte subtypes in each ACC samples. ACC, Adrenocortical carcinoma.

100%

· B cells naive

. B cells memory

· Plasma cells

= T cells CD8

80%

= T cells CD4 naive

= T cells CD4 memory resting

= T cells CD4 memory activated

= T cells follicular helper

= T cells regulatory (Tregs)

Relative Percent

· T cells gamma delta

60%

· NK cells resting

· NK cells activated

= Monocytes

= Macrophages MO

· Macrophages M1

Macrophages M2

40%

# Dendritic cells resting

# Dendritic cells activated

· Mast cells resting

· Mast cells activated

. Eosinophils

· Neutrophils

20%

0%

FCIGA-OR-ASAV-81A-118-A795-83

ICGA-OR-ASLD-014-118-4255-83

TOGE-OR AN.8-014-118-A295.83

TOGA-OR-ASS-01A-118.APPS-83