Supplement Figure 2 related to Figure 4.

A

x-Axis Cell Line Set

ACC NCI plus Surgical

x-Axis Data Type

mda:Signatures, Miscellaneous data

Identifier

ADS

y-Axis Cell Line Set

ACC NCI plus Surgical

y-Axis Data Type

xsq: RNA-seq Expression (log2 FPKM+1)

Identifier

NR5A1

Univariate Analysis / Plot Data NR5A1 (xsq) vs. ADS (mda) Pearson correlation (r)=0.68, p-value=0.03

· CU-ACC1

· CU-ACC2

· H295R

· SW-13

· ACC surgical

CU-ACC1

NR5A1 (xsq, ACC NCI plus Surgical)

7.5

H295R

31

38

33

30

5.0

34

37

CU-ACC2

2.5

SW-13

0.0

-1.0

-0.5

0.0

0.5

1.0

1.5

ADS (mda, ACC NCI plus Surgical)

B

x-Axis Cell Line Set

ACC NCI plus Surgical

x-Axis Data Type

xsq: RNA-seq Expression (log2 FPKM+1)

Identifier

DLK1

y-Axis Cell Line Set

ACC NCI plus Surgical

y-Axis Data Type

xsq: RNA-seq Expression (log2 FPKM+1)

Identifier

IGF2

Univariate Analysis / Plot Data IGF2 (xsq) vs. DLK1 (xsq) Pearson correlation (r)=0.92, p-value=0.00013

12.5

CU-ACC1

31

IGF2 (xsq, ACC NCI plus Surgical)

33

10

34

30

38

37

7.5

H295R

5

CU-ACC2

2.5

0

SW-13

0

5

10

DLK1 (xsq, ACC NCI plus Surgical)

(A) Correlation between the ADS score and the Nuclear Receptor Subfamily 5, Group A, Member 1 NRSA1, which also referred to as Steroidogenic Factor-1 (SF1).

(B) Overexpression of IGF2 in ACC cell lines and tumor samples and correlation with the expression of the surface receptor gene DLK1.